Anti-Von Willebrand Factor antibody (ab11713)

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商品描述

Product name

Anti-Von Willebrand Factor antibody
See all Von Willebrand Factor primary antibodies
Description

Sheep polyclonal to Von Willebrand Factor
Host species

Sheep
Tested applications

Suitable for: IHC-Fr,Immunodiffusion,Flow Cyt,ELISA,ICC/IF
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Human, Pig
Immunogen

Purified human von Willebrand factor.
Positive control
- Tonsil.
General notes

This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: Glycine buffered saline, 0.1% EACA, 0.01% Benzamidine, 0.0292% EDTA
Purity

Ion Exchange Chromatography
Purification notes

Purified IgG was prepared from serum by ion exchange chromatography.
Clonality

Polyclonal
Isotype

IgG

Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody (ab11713)This image is courtesy of an anonymous abreview.

ab11713 at a 1/1000 dilution staining Von Willebrand Factor in mouse blood vessels by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at 19°C. Secondary used at 1/200 polyclonal Donkey anti-sheep conjugated to Alexa Fluor 647 (pink). Counterstain (blue) DAPI.
Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody (ab11713)Image courtesy of an anonymous Abreview.

ab11713 staining Von Willebrand Factor in porcine heart tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked with 10% serum for 30 minutes at 22°C and then incubated with ab11713 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated donkey anti-sheep polyclonal used at a 1/1000 dilution.