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Geneticin Selective Antibiotic (G418 Sulfate) (50 mg/mL)

Geneticin Selective Antibiotic (G418 Sulfate) (50 mg/mL)

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商品描述
Geneticin Selective Antibiotic (G418 Sulfate) (50 mg/mL),10131035,Thermo,Gibco

描述

蒸馏水中 50 mg/ml 活性 Geneticin® 筛选抗生素。 无菌过滤。也成为G418或G-418
应用: Geneticin® 选择抗生素是庆大霉素相关的氨基糖苷类 (1-3),在分子遗传学实验中用作筛选抗生素 (4,5)。 对细菌 (1,2)、酵母 (3,4)、高等植物 (5) 和哺乳动物细胞 (6-8) 以及原生动物和寄生虫 (9,10) 有毒性。 其抗性基因是显性的,位于转座子 Tn601(903)和 Tn5 上。 Tn5 上的基因被称为氨基糖苷磷酸转移酶 3´ (II) 或 APH (3´)II,而 Tn601 上的基因被称为氨基糖苷磷酸转移酶 3fi(I) 或 APH (3´)I。这些基因源自细菌,但可在真核细胞中表达。 将这些基因中的任何一个导入细胞均可以使细胞具有 Geneticin® 筛选抗生素抗性,从而能在含有 Geneticin® 筛选抗生素的培养基中生长。

性质:
比旋 .................. 以水中干品计 104“C 至 121“C
分子式 ........................................................... C20H40o10N4•2H2SO4
分子量................................ 496.5(碱),692.7(硫酸盐)
效力 ..................................................................... >= 700 µg/mg
元素分析................. C=34.68, N=8.09, H=6.40(硫酸盐)
重金属 .........................................................<20 ppm, Pb

稳定性:
-20°C 或 2°C 至 8°C 下在 24 个月内稳定。

注:
选择抗性细胞时培养基中所需的 Geneticin® 筛选抗生素用量各有不同。 扩增中的细胞在 3 至 7 天后出现死亡。

规格:

Validated Application:
Eukaryotic Selection⁄Stable Cell Line Generation
Agent:
Geneticin® (G-418)
Form:
Liquid
Reagent Type:
Antibiotic (Selective)
Concentration:
50 mg⁄mL
Product Line:
Geneticin®
Product Size:
20 mL
Sterility:
Sterile
Shipping Condition:
Room Temperature

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