描述:
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the
isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA,
and protein from a variety of liquid samples. This monophasic solution of
phenol and guanidine isothiocyanate is designed to isolate separate fractions
of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast,
bacterial, and viral origin, typically within one hour.
• Formulated for use with liquid samples such as serum and virus
preparations
• Facilitates recovery of RNA, DNA, and protein from a single liquid
sample
• Offers excellent lysis capability, even with difficult biological fluids
Reliably Purify RNA from Multiple Sample Sources
TRIzol® LS Reagent is designed for processing a variety of liquid samples
of up to 0.25 ml in volume. TRIzol® LS Reagent differs from the standard
TRIzol® Reagent in concentration, which permits larger samples to be processed.
Just as with the standard TRIzol® Reagent, the integrity of resulting RNA
preparations is maintained by the highly effective inhibition of RNase activity
during sample homogenization. The simplicity of the TRIzol® LS Reagent method
allows simultaneous processing of a large number of samples. The entire
procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent
is free of protein and DNA contamination.
Formulated to Serve Multiple Isolations
TRIzol® LS Reagent allows you to perform sequential precipitation of RNA,
DNA, and proteins from a single sample. After homogenizing the sample with
TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to
separate into a clear upper aqueous layer (containing RNA), an interface, and a
red lower organic layer (containing the DNA and proteins). RNA is precipitated
from the aqueous layer with isopropanol. DNA is precipitated from the
aqueous/organic interface with ethanol. Protein is precipitated from the
phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA,
or protein is washed to remove impurities, and then resuspended for use in
downstream applications.
Purified Products are Ideal for Use with a Variety of Applications
Isolated RNA can be used in real-time
quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein
can be used for western blots, recovery of some enzymatic activity, and some immunoprecipitation.
For Research Use Only. Not for human or animal therapeutic or diagnostic
use.
商品类别
|
*商品编号
|
商品名称
|
规格型号
|
Thermo
|
10131035
|
Geneticin
Selective Antibiotic (G418 Sulfate)
|
20ml
|
Thermo
|
10217016
|
dCTP
|
|
Thermo
|
10218014
|
dGTP
|
|
Thermo
|
10296010
|
TRIzol
LS Reagent
|
100ml
|
Thermo
|
10296028
|
LS
Reagent
|
200ML
|
Thermo
|
10362100
|
Cellfectin
II Reagent
|
1ml
|
Thermo
|
10787018
|
1
Kb Plus DNA Ladder
|
250ug
|
Thermo
|
10902104
|
SF-900II
SFM 培养基
|
1000ml
|
Thermo
|
11-889-11
|
高压湿热灭菌指示胶带
|
19mm*55m
|
Thermo
|
11668019
|
Lipofectamine
2000 Transfection Reagent
|
1.5ml
|
Thermo
|
11668027
|
Lipofectamine
2000 Transfection Reagent
|
0.75ml
|
Thermo
|
11668030
|
Lipofectamine
2000 Transfection Reagent
|
0.3ml
|
Thermo
|
11668500
|
Lipofectamine
2000 Transfection Reagent
|
15ml
|
Thermo
|
11965092
|
DMEM
,High Glucose
|
500ML
|
Thermo
|
12935200
|
阴性对照
Stealth siRNA Negative Co
|
1kit
|
Thermo
|
1299003
|
Stealth
RNAi Pre-designed siR
|
1kit
|
Thermo
|
13-379-4
|
耐擦拭记号笔
|
1EA
|
Thermo
|
13-379-5
|
耐擦拭记号笔
|
1EA
|
Thermo
|
13-379-6
|
耐擦拭记号笔
|
1EA
|
Thermo
|
13-379-7
|
耐擦拭记号笔
|
1EA
|
Thermo
|
13-6800
|
Transferrin
Receptor Monoclonal Antibody
|
100ug
|
Thermo
|
13778075
|
RNAiMAX
Transfection Reagent
|
0.75ml
|
Thermo
|
13778150
|
RNAiMAX
Transfection Reagent
|
1.5ml
|
Thermo
|
14750100
|
转染效率对照
Block-IT Alexa Fluor Re
|
1kit
|
Thermo
|
15338-100
|
Lipofectamine
LTX Plus Reag
|
1ml
|
Thermo
|
15596018
|
TRIzol
Reagent
|
200ml
|
Thermo
|
15596026
|
TRIzol
Reagent
|
100ml
|
Thermo
|
174952
|
T75
Nunclon Sphera EasYFlask
|
4个/包,6包/箱
|
Thermo
|
18080085
|
SuperScript
III Reverse Transcriptase
|
4
x 10,000 units
|
Thermo
|
22-362-566
|
肝素钠化毛细管
|
200支/瓶
|
Thermo
|
22-362-574
|
非肝素钠化毛细管
|
200支/瓶
|
Thermo
|
23209
|
Bovine
Serum Albumin Standard
|
10支
|
Thermo
|
26102
|
N-succinimidyl
S-acetylthioacetate
|
50MG
|
Thermo
|
3053835
|
MX35
ULTRA超优型一次性刀片/病理刀片
|
50片
|
Thermo
|
317121001
|
0.1-2.5
μL Junior可调 移液器
|
|
Thermo
|
317121002
|
0.5-10
μL Junior可调 移液器
|
|
Thermo
|
317121003
|
2-20
μL Junior可调 移液器
|
|
Thermo
|
317121004
|
5-50
μLJunior可调 移液器
|
|
Thermo
|
317121005
|
10-100
μLJunior可调 移液器
|
|
Thermo
|
317121006
|
20-200
μL Junior可调 移液器
|
|
Thermo
|
317121007
|
100-1000
μL Junior可调 移液器
|
|
Thermo
|
317121008
|
1000-5000
μL Junior可调 移液器
|
|
Thermo
|
317121009
|
1-10
mL Junior可调 移液器
|
|
Thermo
|
339650
|
15ML
离心管
|
50个
|
Thermo
|
34577
|
SuperSignal
West Pico PLUS Chemiluminescent Substrate
|
200ml
|
Thermo
|
37528
|
Casein
in PBS
|
1L
|
Thermo
|
4115-0125
|
125ML摇菌瓶
|
24个
|
Thermo
|
4306737
|
MicroAmp
Optical 96-Well Reaction Plate with Barcode
|
20
plates
|
Thermo
|
4309849
|
MicroAmp
Optical 384-Well Reaction Plate with Barcode
|
50
plates
|
Thermo
|
4311971
|
MicroAmp
Optical Adhesive Film
|
100
covers
|
Thermo
|
4366596
|
TaqMan
MicroRNA Reverse Transc
|
200R
|
Thermo
|
4440047
|
Master
Mix II, no UNG
|
2
x 5 mL
|
Thermo
|
444202
|
5ML试管
|
100个
|
Thermo
|
4478359
|
Total
Exosome Isolation Reagent (from cell culture media)
|
50ml
|
Thermo
|
4478360
|
Total
Exosome Isolation Reagent
|
6ml
|
Thermo
|
4483354
|
Optical
96-Well Clear Reaction Plates with Barcode
|
20
plates
|
Thermo
|
4640000
|
1-10UL
F3移液器 适配10ul 吸头
|
|
Thermo
|
4640030
|
F3
2-20 μl 单道移液器
|
|
Thermo
|
4640040
|
F3
10-100 μl 单道移液器
|
|
Thermo
|
4640050
|
F3
20-200 μl 单道移液器
|
|
Thermo
|
4640060
|
F3
100-1000 μl 单道移液器
|
|
Thermo
|
4640110
|
F3
0.2-2 μl 单道移液器
|
|
Thermo
|
4641010
|
F1
型0.2-2UL
|
|
Thermo
|
4641060
|
F1
型2-20UL
|
|
Thermo
|
4641080
|
F1型移液器20-200UL
|
200ul
|
Thermo
|
4641100
|
F1
型100-1000UL
|
|
Thermo
|
4642030
|
F2单道可调移液器1-10ul
|
1EA
|
Thermo
|
4642070
|
F2单道可调移液器10-100ul
|
1EA
|
Thermo
|
4642090
|
F2单道可调移液器100-1000ul
|
1EA
|
Thermo
|
5115-0032
|
实验室专用冷却盒
|
1个
|
Thermo
|
62080-00
|
标准实验吸液垫
|
350张
|
Thermo
|
62248
|
DAPI
Solution
|
1ml
|
Thermo
|
62251
|
DRAQ5
Fluorescent Probe Soluti
|
200ul
|
Thermo
|
62254
|
DRAQ5
Fluorescent Probe Solution
|
50ul
|
Thermo
|
711223
|
Desmoplakin
Antibody, ABfinity Rabbit Oligoclonal
|
100ug
|
Thermo
|
78510
|
T-PER
Tissue Protein Extraction Reagent
|
500ml
|
Thermo
|
78835
|
Nuclear
and Cytoplasmic Extraction Reagents
|
75ml
|
Thermo
|
87785
|
Halt
Protease Inhibitor Cocktail, EDTA-Free (100X)
|
1ml
|
Thermo
|
88221
|
HisPur™
Ni-NTA Resin
|
|
Thermo
|
88423
|
IMDM
Media for SILAC
|
500ml/瓶
|
Thermo
|
89842
|
Mem-PER
Plus Membrane Protein Extraction Kit
|
300ml
|
Thermo
|
89874
|
Mitochondria
Isolation Kit for Cultured Cells
|
115ml
|
Thermo
|
9057
|
0.45
滤器(S1配件)
|
25个
|
Thermo
|
940907
|
氟离子标液
|
475ML
|
Thermo
|
940909
|
氟离子强度调节剂
|
3.79L
|
Thermo
|
9501
|
S1白色电动移液器
|
1EA
|
Thermo
|
A-11001
|
Goat
anti-Mouse IgG (H+L) Seco
|
500ul/支
|
Thermo
|
A-11008
|
Goat
anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
|
500ul
|
Thermo
|
A-11016
|
Donkey
anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, AlF594
|
500ul
|
Thermo
|
A-11032
|
Goat
anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,AF594
|
500ul
|
Thermo
|
A-11037
|
Goat
anti-Rabbit IgG AF594
|
500ul
|
Thermo
|
A-11077
|
Goat
anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568
|
|
Thermo
|
A-21036
|
Goat
anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AF700
|
500ul
|
Thermo
|
A-21038
|
Goat
anti-Rabbit IgG Cross-Adsorbed Secondary AF700
|
500ul
|
Thermo
|
A-21121
|
Goat
anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
|
250ul
|
Thermo
|
A-21203
|
Donkey
anti-Mouse IgG (H+L) Se
|
500ul/支
|
Thermo
|
A-21206
|
Donkey
anti-Rabbit IgG (H+L) S
|
500ul/支
|
Thermo
|
A-21235
|
Goat
anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647
|
500ul
|
Thermo
|
A-21447
|
Donkey
anti-Goat IgG (H+L) AF647
|
500ul
|
Thermo
|
A-21448
|
Donkey
anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, AF647
|
500ul
|
Thermo
|
A25742
|
PowerUp
SYBR Green Master Mix
|
5ml
|
Thermo
|
A27012
|
Rabbit
anti-Goat IgG AF488
|
1mg
|
Thermo
|
A27034
|
Goat
anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488
|
1mg
|
Thermo
|
A28175
|
Goat
anti-Mouse IgG (H+L) AF488
|
1mg
|
Thermo
|
A32723
|
Goat
anti-Mouse IgG Highly Cross-Adsorbed Secondary Antibody, AF Plus 488
|
1mg
|
Thermo
|
A32754
|
Donkey
anti-Rabbit IgG Highly Cross-Adsorbed Secondary Antibody, AF Plus 594
|
1mg
|
Thermo
|
AB1771
|
Low
Profile Tubes (white) and Ultra Clear Caps, strips of 8
|
250
strips
|
Thermo
|
AM1334
|
MEGAscript
T7 Transcription Kit
|
40T
|
Thermo
|
AM1340
|
SP6
Transcription Kit
|
25T/盒
|
Thermo
|
AM1561
|
miRNA
Isolation Kit, without phenol
|
40次
|
Thermo
|
AM17121
|
Dye-Labeled
Pre-miR Negative C
|
5nmol
|
Thermo
|
AM1908
|
Transcription
Clean-Up Kit
|
20T
|
Thermo
|
AM2270
|
RNase
A (affinity purified)
|
200ug
|
Thermo
|
AM2271
|
RNase
A (affinity purified)
|
1mg
|
Thermo
|
AM2694
|
SUPERase
In RNase Inhibitor
|
10ug
|
Thermo
|
AM7021
|
RNAlater
Stabilization Solution
|
500ml
|
Thermo
|
AM9260G
|
0.5
M EDTA PH 8.0
|
100ml/瓶
|
Thermo
|
AM9480-100ML
|
LiCl
Precipitation 7.5M
|
100ML
|
Thermo
|
AM9624
|
PBS(10X) pH 7.4
|
500ml
|
Thermo
|
AM9740
|
3M
SODIUM ACETATE PH 5.5
|
100ml
|
Thermo
|
AM9782
|
RNase
Decontamination Solution
|
6瓶
|
Thermo
|
AM9915G
|
DEPC-Treated
Water
|
100ml
|
Thermo
|
AM9938
|
Nuclease-Free
Water (not DEPC-Treated)
|
100ml
|
Thermo
|
C-400
|
Carboxy-H2DCFDA
(general oxida
|
25mg/支
|
Thermo
|
C10329
|
Click-iT
RNA Alexa Fluor 488 I
|
1kit
|
Thermo
|
C10506
|
CellLight
Actin-GFP, BacMam 2.
|
100ul/支
|
Thermo
|
C10509
|
CellLight
Tubulin-GFP, BacMam
|
100ul/支
|
Thermo
|
C10582
|
Actin-GFP,
BacMam 2.0
|
1ml
|
Thermo
|
C10600
|
CellLight
Mitochondria-GFP, BacMam 2.0
|
1ml
|
Thermo
|
C10601
|
Mitochondria-RFP,
BacMam 2.0
|
1ml
|
Thermo
|
C10603
|
CellLight
Nucleus-RFP, BacMam 2.0
|
1ml
|
Thermo
|
C10613
|
Tubulin-GFP,
BacMam 2.0
|
1ml/包
|
Thermo
|
C2925
|
CellTracker
Green CMFDA Dye
|
1mg
|
Thermo
|
C3100MP
|
Calcein,
AM - Special Packagin
|
20支/包
|
Thermo
|
C34552
|
CellTracker
Red CMTPX Dye
|
20*50ug
|
Thermo
|
C34778
|
Cholera
Toxin Subunit B (Recombinant), Alexa Fluor 647
|
100ug
|
Thermo
|
C35011
|
CyQUANT
Direct Cell Proliferat
|
10
microplates/包
|
Thermo
|
C7025
|
CellTracker
Green CMFDA Dye
|
20*50ug
|
Thermo
|
E-10345
|
EU(5-Ethynyl
Uridine)
|
5mg/支
|
Thermo
|
E106172-100g
|
1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐
|
100g
|
Thermo
|
EHVWF
|
vWF
ELISA Kit, Human
|
96T
|
Thermo
|
EN0541
|
RNase
T1
|
100000U/支
|
Thermo
|
EXPERTCTS
|
TDS水质测试笔
|
1EA
|
Thermo
|
FD0594
|
FastDigest
NotI
|
50
reactions
|
Thermo
|
FD0684
|
FastDigest
XbaI
|
300
reactions
|
Thermo
|
FD0694
|
FastDigest
XhoI
|
400
reactions
|
Thermo
|
FD1034
|
FastDigest
KspAI
|
50
reactions
|
Thermo
|
H3570
|
Hoechst
33342
|
10ml
|
Thermo
|
K1622
|
RevertAid
First Strand cDNA Synthesis Kit
|
100
reactions
|
Thermo
|
L3000001
|
Lipofectamine
3000 Transfection Reagent
|
0.1ml
|
Thermo
|
L3000008
|
Lipofectamine
3000 Transfection Reagent
|
0.75ml
|
Thermo
|
L3000015
|
Lipofectamine
3000 Transfection Reagent
|
1.5ml
|
Thermo
|
L34975
|
Fixable
Near-IR Dead Cell Stain Kit
|
80assays
|
Thermo
|
Locator
4 plus
|
液氮罐
|
1台
|
Thermo
|
M34152
|
MitoProbe?
JC-1 Assay Kit
|
1kit
|
Thermo
|
M36008
|
Red
Mitochondrial Superoxide Indicator
|
10x50ug
|
Thermo
|
M7512
|
MitoTracker
Red CMXRos
|
1kit
|
Thermo
|
MA5-23597
|
Human
IL-1 beta Antibody APC
|
100T
|
Thermo
|
N8010560
|
MicroAmp
Optical 96-Well Reaction Plate
|
10
plates
|
Thermo
|
N8080119
|
RNase抑制剂
|
2000U/支
|
Thermo
|
P-36931
|
ProLong
Gold Antifade Reagent
|
10ml/瓶
|
Thermo
|
P36931
|
Gold
Antifade Mountant with DAPI
|
10ml
|
Thermo
|
P36941
|
ProLong
Gold Antifade Mountant
|
2ml/支
|
Thermo
|
PA1-310B
|
Estrogen
Receptor beta Polyclonal Antibody
|
100ug
|
Thermo
|
PA5-29013
|
EEA1
Polyclonal Antibody
|
100ul
|
Thermo
|
PA5-36028
|
Phospho-SMAD2/SMAD3
(Thr8) Polyclonal Antibody
|
100ul
|
Thermo
|
PA5-36029
|
Phospho-SMAD2
(Thr220) Polyclonal Antibody
|
100ul
|
Thermo
|
PA5-36877
|
Phospho-SMAD3
(Ser204) Polyclonal Antibody
|
100ul
|
Thermo
|
PA5-37635
|
Phospho-SMAD2
(Thr220) Polyclonal Antibody
|
100ul
|
Thermo
|
PA5-79598
|
LHR
Polyclonal Antibody
|
100ug
|
Thermo
|
PHC9514
|
TPO
(Thrombopoietin), Recombin
|
10ug
|
Thermo
|
Q32851
|
dsDNA
HS Assay Kit
|
100
assays
|
Thermo
|
Q32853
|
dsDNA
BR Assay Kit
|
500
assays
|
Thermo
|
Q32854
|
dsDNA
HS Assay Kit
|
500
assays
|
Thermo
|
R25005
|
Zeocin
Selection Antibiotic
|
50ML
|
Thermo
|
S33102
|
Safe
DNA Gel Stain
|
400ul
|
Thermo
|
S36938
|
Gold
Antifade Mountant with DAPI
|
10ml
|
Thermo
|
T-668
|
Tetramethylrhodamine,
Methyl E
|
25mg/瓶
|
Thermo
|
T3168
|
JC-1
Dye (Mitochondrial Membra
|
5mg/支
|
Thermo
|
T668
|
TMRM
|
25mg
|
Thermo
|
T7471
|
Texas
Red X Phalloidin
|
300
units
|
Thermo
|
W11261
|
Wheat
Germ Agglutinin, AF488
|
5mg
|
Thermo
|
ZY00100BOX
|
10%
Zymogram Plus (Gelatin) Protein Gels
|
10块
|
Thermo
|
am1333
|
MEGAscript
T7 Kit
|
25T
|