English 简体中文
产品
文章
搜索
搜索
首页 ∷ 试剂 ∷ 近岸蛋白 ∷ Recombinant Human VEGF165(Cat. No.:C083)
当前位置：
Recombinant Human VEGF165(Cat. No.:C083)

Recombinant Human VEGF165(Cat. No.:C083)

Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

0.00
0.00
  
商品描述

Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

产品描述
Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.
蛋白编号
P15692-4
产品别称
Vascular Endothelial Growth Factor Isoform 165; VEGF165
分子量
19.1 KDa
表观分子量
18-22 KDa, reducing conditions
剂型描述
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
内毒素
Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
纯度
Greater than 95% as determined by reducing SDS-PAGE.
生物活性
Loaded Human VEGFR1-Fc(Cat#CJ93) on Protein A Biosensor, can bind Human VEGF165(Cat#C083) with an affinity constant of 0.29 nM as determined in BLI assay. 
Loaded Human VEGF-165 Antibody(12E3)-mFc(Cat#DA019) on AMQ Biosensor, can bind Human VEGF165(Cat#C083) with an affinity constant of <10-3 n M as determined in BLI assay. 
Loaded Human VEGF-165 Antibody(8D7)-mFc(Cat#DA020) on AMQ Biosensor, can bind Human VEGF165(Cat#C083) with an affinity constant of 54.9 nM as determined in BLI assay. 
Loaded Human VEGF-165 Antibody(10F9)-mFc(Cat#DA021) on AMQ Biosensor, can bind Human VEGF165(Cat#C083) with an affinity constant of 0.004 n M as determined in BLI assay. 
Loaded Human VEGF Antibody (5E7)-Fc(Cat#DA082) on AHC Biosensor, can bind Human VEGF165(Cat#C083) with an affinity constant of <10-3 nM as determined in BLI assay.
Loaded Human VEGF-165 Antibody (6D9)-Fc(Cat#DA083) on AHC Biosensor, can bind Human VEGF165(Cat#C083) with an affinity constant of <10-3 nM as determined in BLI assay.
Immobilized Human VEGF165(Cat#C083) at 2μg/ml (100 μl/well) can bind Human VEGF165 antibody(Cat#DA082).The ED50 of Human VEGF165 antibody(Cat#DA082) is 1.44 ng/ml.
复溶

  • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

  • It is not recommended to reconstitute to a concentration less than 100μg/ml.

  • Dissolve the lyophilized protein in distilled water.

  • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

保存条件

  • Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

  • Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

  • Aliquots of reconstituted samples are stable at < -20°C for 3 months.

运输条件

  • The product is shipped at ambient temperature.

  • Upon receipt, store it immediately at the temperature listed below.

产品背景
Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
仅供科研或生产使用，不可直接应用于人体。