Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).
-
Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument
-
Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)
-
Amplify genomic DNA and difficult amplicons
-
Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
Instrument Compatibility
iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
|
Systems
|
|
Applied Biosystems
|
-
StepOne/StepOnePlus
-
7500, ViiA 7
-
7000, 7300, 7700, 7900HT, QuantStudio Systems
|
|
Stratagene
|
|
|
Eppendorf
|
-
Mastercycler ep realplex 2, 4
|
|
QIAGEN
|
-
Corbett Rotor-Gene 3000, 6000, Q
|
|
Roche
|
-
LightCycler 480, 96
-
LightCycler 1.0, 1.5, 2.0*
|
|
BioFire
|
-
LightScanner HR-1
-
LightScanner 32*
|
* BSA must be added according to instrument specifications
Packaging Options
|
Number of 20 µl Reactions
|
Supermix Volume, ml
|
Catalog Number
|
|
200
|
2 x 1
|
1725120
|
|
500
|
5 x 1
|
1725121
|
|
1,000
|
10 x 1
|
1725122
|
|
2,500
|
5 x 5
|
1725124
|
|
5,000
|
10 x 5
|
1725125
|
See the iTaq Universal SYBR Green Supermix product page for more information and documentation.
Related Products
-
View all Real-Time PCR Supermixes and Kits
-
PrimePCR™ PCR Primers & Assays
Related Categories
-
PCR and qPCR Reagents
-
Real-Time PCR Detection Systems
-
PCR Plastic Consumables
-
Instrument Validation Tools and Services
Supporting Documents
-
iTaq™ Universal SYBR® Green Supermix Product Insert
-
Amplification Plastics and Reagents Selection Guide
Online Resources
-
qPCR/Real-Time PCR
-
Genomics
