useful pH range 6.8 - 8.2 pKa (25 °C) 7.5分子生物学级用于用T4 RNA连接酶进行RNA3' -末端标记，分离与分析核RNA之反应缓冲液组分、预杂交缓冲液缓和杂交缓冲液成分
useful pH range 6.8 - 8.2 pKa (25 °C) 7.5
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37°C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.
相关属性
| CAS编号 | 7365-45-9 |
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| 敏感性 | 对湿度敏感 |
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| 熔点 | 210 °C |
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| 溶解性 | 溶于water, 最高浓度 (mg/mL): 238.3, 最高浓度(mM): 1000 |
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| 储存温度 | 充氩 |
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| Reaxys-RN | 883043 |
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| RTECS | TL6809000 |
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| MDL号 | MFCD00006158 |
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| 密度 | 1.07 |
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| 分子量 | 238.3 |
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| 分子式 | C8H18N2O4S |
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| EC号 | 230-907-9 |
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| 品牌 | 阿拉丁 |
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