General description:

Triton™ X-100是一种常用的非离子型的表面活性剂和乳化剂，经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂，并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。

Application:

Triton™ X-100已用于：

• 免疫组化用于平面视网膜的染色

• 与冰冷的PBS（磷酸盐缓冲液）一起悬浮细胞用于细胞DNA分析和Annexin V检测

• 在免疫荧光显微研究中对细胞进行通透化处理

• 在LDH检测中作为阳性对照用于测定细胞膜的完整性

• 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性

• 外层膜蛋白提取物的制备

• 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆

• 免疫荧光标记的组织切片处理

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection

Application

Triton™ X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas

• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay

• To permeabilise cells during Immunofluorescent microscopic studies

• As a positive control in LDH assay to determine the cell membrane integrity

• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure

• For the preparation of outer membrane protein exctract

• As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells

• In the treatment of tissue sections for Immunofluorescence labeling

相关属性