产品名称
Rat Afp(Alpha-fetoprotein) ELISA Kit
货号
ER0074
别称
Afp ELISA Kit, αFP ELISA Kit, Alpha-Fetoprotein ELISA Kit, AFP ELISA Kit, FETA ELISA Kit, HPAFP ELISA Kit, oralpha fetal protein ELISA Kit
检测方法
Sandwich ELISA, Double Antibody
应用
Afp ELISA Kit allows for the in vitro quantitative determination of α-FP concentrations in serum, plasma, tissue homogenates and other biological fluids.
规格
48T/96T
检测范围
0.313-20ng/ml
灵敏度
< 0.188ng/ml
种属
Rat
UniProt ID
P02773
标准曲线
存储
2-8 ℃ for 6 months
回收率
Matrices listed below were spiked with certain level of α-FP and the recovery rates were calculated by comparing the measured value to the expected amount of α-FP in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|
| serum(n=5) | 87-100 | 93 |
| EDTA plasma(n=5) | 88-99 | 91 |
| heparin plasma(n=5) | 88-97 | 93 |
线性度
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of α-FP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
|---|
| serum(n=5) | 85-97% | 89-99% | 85-97% |
| EDTA plasma(n=5) | 83-98% | 82-101% | 85-97% |
| heparin plasma(n=5) | 85-99% | 81-99% | 80-91% |
变异系数（%）
批内差：CV<8%
批间差：CV<10%
注意事项
仅供科研使用
