产品名称
Rat AKR1B1(Aldose reductase) ELISA Kit
货号
ER1597
别称
AKR1B10 ELISA Kit, Aldo-keto reductase family 1 member B10 ELISA Kit, Small intestine reductase ELISA Kit, SI reductase ELISA Kit, Aldose reductase-related protein ELISA Kit, ARP ELISA Kit, hARP ELISA Kit, ARL-1 ELISA Kit, Aldose reductase-like ELISA Kit
检测方法
Sandwich ELISA, Double Antibody
应用
AKR1B1 ELISA Kit allows for the in vitro quantitative determination of AKR1B1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
规格
48T/96T
检测范围
0.313-20ng/ml
灵敏度
< 0.188ng/ml
种属
Rat
UniProt ID
P07943
标准曲线

存储
2-8 ℃ for 6 months
回收率
Matrices listed below were spiked with certain level of AKR1B1 and the recovery rates were calculated by comparing the measured value to the expected amount of AKR1B1 in samples.
Matrix | Recovery range(%) | Average(%) |
---|
serum(n=5) | 85-93 | 91 |
EDTA plasma(n=5) | 91-102 | 96 |
heparin plasma(n=5) | 91-102 | 96 |
线性度
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKR1B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
---|
serum(n=5) | 85-91% | 85-101% | 87-93% |
EDTA plasma(n=5) | 83-98% | 82-91% | 84-101% |
heparin plasma(n=5) | 88-98% | 84-89% | 80-98% |
变异系数(%)
批内差:CV<8%
批间差:CV<10%
注意事项
仅供科研使用