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Anti-VEGF Receptor 2 antibody (ab11939)

Anti-VEGF Receptor 2 antibody (ab11939)

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商品描述
  • Product name
    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 2
  • Host species
    Rabbit
  • Specificity
    The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types.
  • Tested applications
    Suitable for: Sandwich ELISA,ELISA,WB,Flow Cyt,IHC-Fr,IP,Neutralising
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Constituent: PBS
  • Concentration
    • 100 µg at 1 mg/ml

  • Purity
    Protein A purified
  • Purification notes
    This antibody is affinity purified from rabbit sera.
  • Clonality
    Polyclonal
  • Isotype
    IgG

  • Western blot - Anti-VEGF Receptor 2 antibody (ab11939)
    Western blot - Anti-VEGF Receptor 2 antibody (ab11939)
    All lanes : Anti-VEGF Receptor 2 antibody (ab11939)

    Lane 1 : recombinant human soluble VEGFR-2
    Lane 2 : recombinant native VEGFR-2

    Secondary
    All lanes : anti-rabbit AP-conjugated antibody

    Predicted band size: 151 kDa



    Samples were loaded in 10% SDS-PAGE under reducing conditions.


  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)
    Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.


  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)
    Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.


  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
    Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.


  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)
    Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.


  • Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)
    Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)
    Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.



Compatible Secondaries
 
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) 
Goat Anti-Rabbit IgG H&L (HRP) (ab205718) 
Conjugation kits
 
PE / R-Phycoerythrin Conjugation Kit (ab102918) 
APC Conjugation Kit (ab201807) 
Isotype control
 
Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870) 
sELISA pair antibody
 
Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)