abcam-广州市百菱生物科技有限公司

Anti-VEGF Receptor 2 antibody (ab11939)

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商品描述

Product name

Anti-VEGF Receptor 2 antibody
See all VEGF Receptor 2 primary antibodies
Description

Rabbit polyclonal to VEGF Receptor 2
Host species

Rabbit
Specificity

The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types.
Tested applications

Suitable for: Sandwich ELISA,ELISA,WB,Flow Cyt,IHC-Fr,IP,Neutralising
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Constituent: PBS
Concentration
- 100 µg at 1 mg/ml
Purity

Protein A purified
Purification notes

This antibody is affinity purified from rabbit sera.
Clonality

Polyclonal
Isotype

IgG

Western blot - Anti-VEGF Receptor 2 antibody (ab11939)

All lanes : Anti-VEGF Receptor 2 antibody (ab11939)

Lane 1 : recombinant human soluble VEGFR-2
Lane 2 : recombinant native VEGFR-2

Secondary
All lanes : anti-rabbit AP-conjugated antibody

Predicted band size: 151 kDa

Samples were loaded in 10% SDS-PAGE under reducing conditions.
Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.
Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.
Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)

Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.