Synthetic peptide corresponding to Mouse Tet2 aa 1600-1700 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q4JK59
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab124297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Secondary antibody - goat anti-rabbit HRP (ab97051)
ICC/IF image of Anti-Tet2 antibody (ab124297) stained WT and Tet2-/- mouse ES cells. The cells were fixed in PFA, permeabilized using 0.25% Triton X-100, and blocked with 10% BSA for 30 minutes. The cells were then incubated with ab124297 at a 1/400 dilution for 13 hours and 20 minutes at 4oC. The secondary antibody was a Rhodamine Red-X AffiniPure Donkey anti-Rabbit used at a 1/500 dilution.
ICC/IF image of Anti-Tet2 antibody (ab124297) stained D3 mouse ES cells. The cells were fixed in 4% PFA, permeabilized using 0.1% Triton X-100, and blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes. The cells were then incubated with ab124297 at a 1/100 dilution for 2 hours at RT. The secondary antibody was a Goat polyclonal Secondary Antibody to Rabbit IgG – H&L Alexa Fluor 488 (ab150077) used at a 1/200 dilution.
Secondary antibody - goat anti-rabbit Alexa 488 (ab150077)
Immunoprecipitation of Tet2, using WT and Tet2 KO mouse ES cells. The protein was immunoprecipitated using 300µg whole cell extract, and 3µg of Rabbit polyclonal to Tet2 (ab124297). The same Tet2 antibody was used for the western blot. Tet2 KO mouse ES cells were used as a negative control.
Band: 223kDa: Tet2.
Compatible
Secondaries
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) |
Goat Anti-Rabbit IgG H&L (HRP) (ab205718) |
Isotype
control
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Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870) |