ELISA Kit

小鼠白介素18(IL-18)ELISA Kit

Mouse Interleukin 18,IL-18 ELISA Kit

CSB-E04609m

IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18

96T

3200

Mus musculus (Mouse)

interleukin 18 (interferon-gamma-inducing factor)

IL18

1.56 pg/mL-100 pg/mL

0.39 pg/mL

1-5h

50-100ul

450 nm

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 100 2.646 2.552 2.599 2.484 50 2.218 2.186 2.202 2.087 25 1.874 1.804 1.839 1.724 12.5 1.332 1.329 1.331 1.216 6.25 0.925 0.901 0.913 0.798 3.12 0.527 0.499 0.513 0.398 1.56 0.261 0.278 0.270 0.155 0 0.119 0.111 0.115

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-94
1:2		Average %		97
		Range %		91-103
1:4		Average %		93
		Range %		88-99
1:8		Average %		94
		Range %		90-100

The recovery of mouse IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-100
EDTA plasma (n=4)		99		94-107

quantitative

Immunology