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首页 ∷ 试剂 ∷ CUSABIO ∷ 人胎盘碱性磷酸酶(PLAP)ELISA Kit CSB-E09160h
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人胎盘碱性磷酸酶(PLAP)ELISA Kit CSB-E09160h

人胎盘碱性磷酸酶(PLAP)ELISA Kit CSB-E09160h

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商品描述

  • 产品类型:
    ELISA Kit
  • 产品名称:

    人胎盘碱性磷酸酶(PLAP)ELISA Kit

  • 英文名称:

    Human Placental alkaline phosphatase,PLAP ELISA Kit

  • 货号:
    CSB-E09160h
  • 别名:
    ALP, FLJ61142, PALP, PLAP, alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Homo sapiens (Human)
  • 待测物名称:
    alkaline phosphatase, placental (Regan isozyme)
  • 缩写:
    ALPP
  • 检测范围:
    84 pg/mL-2000 pg/mL
  • 灵敏度:
    34 pg/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.012 1.983 1.998 1.952  
    1000 1.338 1.341 1.340 1.294  
    500 0.775 0.828 0.802 0.756  
    167 0.357 0.349 0.353 0.307  
    84 0.224 0.235 0.230 0.184  
    0 0.047 0.045 0.046  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-98  
    1:2 Average % 96  
    Range % 88-100  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 97  
    Range % 91-101
  • 回收率:
    The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-104  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 定性/定量:
    quantitative
  • 研究领域:
    Tags & Cell Markers