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Human/Mouse TIAM1 Antibody
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商品描述
Human/Mouse TIAM1 Antibody ，AF5038，R&D：
Human/Mouse TIAM1 Antibody Summary
Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse TIAM1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TIAM1
Met1-Ala378
Accession # Q13009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Western Blot 
View Larger
Detection of Human/Mouse TIAM1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HCT‑116 human colorectal carcinoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
RB01
Contact your distributor for availability.
2 mL / Please Inquire
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
-
12 months from date of receipt, -20 to -70 °C as supplied.
-
1 month, 2 to 8 °C under sterile conditions after reconstitution.
-
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse TIAM1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TIAM1
Met1-Ala378
Accession # Q13009
Met1-Ala378
Accession # Q13009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Western Blot View Larger
View Larger
Detection of Human/Mouse TIAM1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HCT‑116 human colorectal carcinoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
RB01
Contact your distributor for availability.
2 mL / Please Inquire
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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