Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Phospho-Paxillin (Y31) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Phospho-Paxillin (Y31) at approximately 65 to 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Paxillin in HUVEC Human Cells. Paxillin phosphorylated at Y31 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Phospho-Paxillin (Y31) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB61641) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and focal adhesions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
商品类别
|
*商品编号
|
商品名称
|
规格型号
|
R/RD
|
1315-ND-025
|
Recombinant
Mouse Nodal Protein
|
25ug
|
R/RD
|
1315-ND-025/CF
|
Recombinant
Mouse Nodal Protein, CF
|
25ug
|
R/RD
|
201-LB-005
|
Recombinant
Human IL-1 beta/IL-1F2 Protein
|
5ug
|
R/RD
|
220-BB-010
|
Recombinant
Human PDGF-BB Protein
|
10ug
|
R/RD
|
231-BC-025
|
Recombinant
Human FGF acidic Protein
|
25ug
|
R/RD
|
3218-ND-025/CF
|
Recombinant
Human Nodal Protein, CF
|
25ug
|
R/RD
|
3410/10
|
BMS
961
|
10mg
|
R/RD
|
383-CD-050
|
Recombinant
Human CD14 Protein
|
50ug
|
R/RD
|
623-AN-025
|
Recombinant
Human Angiopoietin-2 Protein
|
25ug
|
R/RD
|
6445-LP-025
|
Recombinant
Human LBP (Histidine-tagged) Protein
|
25ug
|
R/RD
|
6635-LP-025
|
Recombinant
Mouse LBP Protein
|
25ug
|
R/RD
|
923-AN-025
|
Recombinant
Human Angiopoietin-1 Protein
|
25ug
|
R/RD
|
982-CD-100
|
Recombinant
Mouse CD14 Fc Chimera Protein, CF
|
100ug
|
R/RD
|
AB-20-NA
|
Human PDGF Polyclonal
Ab
|
1mg
|
R/RD
|
AF1086
|
Human
PD-1 Antibody
|
100ug
|
R/RD
|
AF2685-SP
|
Human
Phospho-Src Antibody
|
25ug
|
R/RD
|
AF3158
|
Mouse
KLF4 Antibody
|
100ug
|
R/RD
|
AF364-SP
|
Human
CCL17/TARC Antibody
|
25ug
|
R/RD
|
AF5038
|
Human/Mouse
TIAM1 Antibody
|
100ug
|
R/RD
|
ARY003B
|
Proteome
Profiler Human Phospho-Kinase Array Kit
|
1kit
|
R/RD
|
BBE6
|
Human
P-Selectin/CD62P ELISA Kit
|
96T
|
R/RD
|
DADT130
|
Human
ADAMTS13 Quantikine ELISA Kit
|
96T
|
R/RD
|
DCD310
|
Human
CD31/PECAM-1 Quantikine ELISA Kit
|
96T
|
R/RD
|
DCD540
|
Human
ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
|
96T
|
R/RD
|
E-305-025
|
Recombinant
Human Ubiquitin Activating Enzyme (UBE1), CF
|
25UG
|
R/RD
|
E2-607-100
|
Recombinant
Human UbcH2/UBE2H Protein, CF
|
100ug
|
R/RD
|
HAF007
|
Mouse
IgG HRP-conjugated Antibody
|
1ml
|
R/RD
|
HS600B
|
Human
IL-6 Quantikine HS ELISA
|
96T
|
R/RD
|
IC1420P
|
Human
alpha Smooth Muscle Acti
|
100T
|
R/RD
|
KGE011
|
Thromboxane
B2 Parameter Assay Kit
|
96T
|
R/RD
|
LXSAHM-16
|
Human
Magnetic Luminex Screening Assay
|
16
PLEX
|
R/RD
|
MAB1205-SP
|
Human/Mouse/Rat
Phospho-JNK Antibody
|
25ul
|
R/RD
|
MAB1310-SP
|
Human
u-Plasminogen Activator
|
25ug
|
R/RD
|
MAB1778-SP
|
Human
Integrin beta 1/CD29 An
|
25ug
|
R/RD
|
MAB2018
|
Human/Mouse
SOX2 MAb
|
100
ug
|
R/RD
|
MAB2076-SP
|
Human/Mouse/Rat
JNK1/JNK2 Antibody
|
25ug
|
R/RD
|
MAB3389-SP
|
Human/Mouse/Rat
Src Antibody
|
25ug
|
R/RD
|
MAB4259-SP
|
Human
Paxillin Antibody
|
25ug
|
R/RD
|
MAB4467-SP
|
Human
FAK Antibody
|
25ug
|
R/RD
|
MAB61641-SP
|
Human
Phospho-Paxillin (Y31) A
|
25ug
|
R/RD
|
MAB7298-SP
|
Human
Phospho-FAK (S843) Antib
|
25ug
|
R/RD
|
MAB807-SP
|
Human
uPAR Antibody
|
25ug
|
R/RD
|
NB100-65392PE
|
P-Selectin/CD62P
Antibody (Pse
|
100T
|
R/RD
|
NBP1-43415APC
|
Integrin
alpha 2b/CD41 Antibod
|
100T
|
R/RD
|
SK-10
|
Ubiquitin
Conjugation Reaction Buffer Kit
|
1kit
|
R/RD
|
U-100H-10M
|
Recombinant
Human Ubiquitin Protein, CF
|
10mg
|
R/RD
|
VAL601
|
Mouse
IL-1 beta Valukine ELISA Kit
|
96T
|