Species Reactivity

Human

Specificity

Detects human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain in direct ELISAs and Western blots. In Western blots under reducing conditions, it reacts with the catalytic domain (B chain) only. In Western blots under non-reducing conditions, it reacts with the B chain and disulfide bond-linked B and A chains. In direct ELISAs, no cross-reactivity with recombinant human (rh) Factor X, rhHGFA, rhKLK‑3, -4, -5, -6, -8, -10, -11, rhThrombin, or recombinant mouse WIF-1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 204212

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain
Ser21-Leu431
Accession # P00749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE), see our available Western blot detection antibodies

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL 

Use in combination with:

Detection Reagent: Human u-Plasminogen Activator (uPA)/Urokinase Biotinylated Antibody (Catalog # BAF1310)

Standard: Recombinant Human u-Plasminogen Activator/Urokinase, CF (Catalog # 1310-SE)

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• 12 months from date of receipt, -20 to -70 °C as supplied.
• 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• 6 months, -20 to -70 °C under sterile conditions after reconstitution.