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Human Integrin beta 1/CD29 Antibody

Human Integrin beta 1/CD29 Antibody

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商品描述

Human Integrin  beta 1/CD29 Antibody，MAB1778-SP,R&D：

Human Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 4B7R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Ocular melanoma cell line V+B2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Integrin  beta 1/CD29 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human skin cancer tissue
Immunoprecipitation
Marshall, J.F. et al. (1998) Br. J. Cancer 77:522.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Integrin  beta 1/CD29 in Human PBMCs by Flow Cytometry. View Larger

Detection of Integrin  beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry Integrin  beta 1/CD29 in MG‑63 Human Cell Line. View Larger

Integrin  beta 1/CD29 in MG‑63 Human Cell Line. Integrin  beta 1/CD29 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Anti­body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti­body (yellow; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.


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