Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Paxillin by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Paxillin in Detroit 551 Human Cell Line. Paxillin was detected in immersion fixed Detroit 551 human embryonic skin fibroblast cell line using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Paxillin in HUVEC Human Cells. Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips. View our protocol for Fluorescent ICC Staining of Cells on Coverslips
商品类别
|
*商品编号
|
商品名称
|
规格型号
|
R/RD
|
1315-ND-025
|
Recombinant
Mouse Nodal Protein
|
25ug
|
R/RD
|
1315-ND-025/CF
|
Recombinant
Mouse Nodal Protein, CF
|
25ug
|
R/RD
|
201-LB-005
|
Recombinant
Human IL-1 beta/IL-1F2 Protein
|
5ug
|
R/RD
|
220-BB-010
|
Recombinant
Human PDGF-BB Protein
|
10ug
|
R/RD
|
231-BC-025
|
Recombinant
Human FGF acidic Protein
|
25ug
|
R/RD
|
3218-ND-025/CF
|
Recombinant
Human Nodal Protein, CF
|
25ug
|
R/RD
|
3410/10
|
BMS
961
|
10mg
|
R/RD
|
383-CD-050
|
Recombinant
Human CD14 Protein
|
50ug
|
R/RD
|
623-AN-025
|
Recombinant
Human Angiopoietin-2 Protein
|
25ug
|
R/RD
|
6445-LP-025
|
Recombinant
Human LBP (Histidine-tagged) Protein
|
25ug
|
R/RD
|
6635-LP-025
|
Recombinant
Mouse LBP Protein
|
25ug
|
R/RD
|
923-AN-025
|
Recombinant
Human Angiopoietin-1 Protein
|
25ug
|
R/RD
|
982-CD-100
|
Recombinant
Mouse CD14 Fc Chimera Protein, CF
|
100ug
|
R/RD
|
AB-20-NA
|
Human PDGF Polyclonal
Ab
|
1mg
|
R/RD
|
AF1086
|
Human
PD-1 Antibody
|
100ug
|
R/RD
|
AF2685-SP
|
Human
Phospho-Src Antibody
|
25ug
|
R/RD
|
AF3158
|
Mouse
KLF4 Antibody
|
100ug
|
R/RD
|
AF364-SP
|
Human
CCL17/TARC Antibody
|
25ug
|
R/RD
|
AF5038
|
Human/Mouse
TIAM1 Antibody
|
100ug
|
R/RD
|
ARY003B
|
Proteome
Profiler Human Phospho-Kinase Array Kit
|
1kit
|
R/RD
|
BBE6
|
Human
P-Selectin/CD62P ELISA Kit
|
96T
|
R/RD
|
DADT130
|
Human
ADAMTS13 Quantikine ELISA Kit
|
96T
|
R/RD
|
DCD310
|
Human
CD31/PECAM-1 Quantikine ELISA Kit
|
96T
|
R/RD
|
DCD540
|
Human
ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
|
96T
|
R/RD
|
E-305-025
|
Recombinant
Human Ubiquitin Activating Enzyme (UBE1), CF
|
25UG
|
R/RD
|
E2-607-100
|
Recombinant
Human UbcH2/UBE2H Protein, CF
|
100ug
|
R/RD
|
HAF007
|
Mouse
IgG HRP-conjugated Antibody
|
1ml
|
R/RD
|
HS600B
|
Human
IL-6 Quantikine HS ELISA
|
96T
|
R/RD
|
IC1420P
|
Human
alpha Smooth Muscle Acti
|
100T
|
R/RD
|
KGE011
|
Thromboxane
B2 Parameter Assay Kit
|
96T
|
R/RD
|
LXSAHM-16
|
Human
Magnetic Luminex Screening Assay
|
16
PLEX
|
R/RD
|
MAB1205-SP
|
Human/Mouse/Rat
Phospho-JNK Antibody
|
25ul
|
R/RD
|
MAB1310-SP
|
Human
u-Plasminogen Activator
|
25ug
|
R/RD
|
MAB1778-SP
|
Human
Integrin beta 1/CD29 An
|
25ug
|
R/RD
|
MAB2018
|
Human/Mouse
SOX2 MAb
|
100
ug
|
R/RD
|
MAB2076-SP
|
Human/Mouse/Rat
JNK1/JNK2 Antibody
|
25ug
|
R/RD
|
MAB3389-SP
|
Human/Mouse/Rat
Src Antibody
|
25ug
|
R/RD
|
MAB4259-SP
|
Human
Paxillin Antibody
|
25ug
|
R/RD
|
MAB4467-SP
|
Human
FAK Antibody
|
25ug
|
R/RD
|
MAB61641-SP
|
Human
Phospho-Paxillin (Y31) A
|
25ug
|
R/RD
|
MAB7298-SP
|
Human
Phospho-FAK (S843) Antib
|
25ug
|
R/RD
|
MAB807-SP
|
Human
uPAR Antibody
|
25ug
|
R/RD
|
NB100-65392PE
|
P-Selectin/CD62P
Antibody (Pse
|
100T
|
R/RD
|
NBP1-43415APC
|
Integrin
alpha 2b/CD41 Antibod
|
100T
|
R/RD
|
SK-10
|
Ubiquitin
Conjugation Reaction Buffer Kit
|
1kit
|
R/RD
|
U-100H-10M
|
Recombinant
Human Ubiquitin Protein, CF
|
10mg
|
R/RD
|
VAL601
|
Mouse
IL-1 beta Valukine ELISA Kit
|
96T
|