Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FAK by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh‑7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
FAK in Human Brain. FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human FAK Monoclonal Antibody (Catalog # MAB4467) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human FAK Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
商品类别
|
*商品编号
|
商品名称
|
规格型号
|
R/RD
|
1315-ND-025
|
Recombinant
Mouse Nodal Protein
|
25ug
|
R/RD
|
1315-ND-025/CF
|
Recombinant
Mouse Nodal Protein, CF
|
25ug
|
R/RD
|
201-LB-005
|
Recombinant
Human IL-1 beta/IL-1F2 Protein
|
5ug
|
R/RD
|
220-BB-010
|
Recombinant
Human PDGF-BB Protein
|
10ug
|
R/RD
|
231-BC-025
|
Recombinant
Human FGF acidic Protein
|
25ug
|
R/RD
|
3218-ND-025/CF
|
Recombinant
Human Nodal Protein, CF
|
25ug
|
R/RD
|
3410/10
|
BMS
961
|
10mg
|
R/RD
|
383-CD-050
|
Recombinant
Human CD14 Protein
|
50ug
|
R/RD
|
623-AN-025
|
Recombinant
Human Angiopoietin-2 Protein
|
25ug
|
R/RD
|
6445-LP-025
|
Recombinant
Human LBP (Histidine-tagged) Protein
|
25ug
|
R/RD
|
6635-LP-025
|
Recombinant
Mouse LBP Protein
|
25ug
|
R/RD
|
923-AN-025
|
Recombinant
Human Angiopoietin-1 Protein
|
25ug
|
R/RD
|
982-CD-100
|
Recombinant
Mouse CD14 Fc Chimera Protein, CF
|
100ug
|
R/RD
|
AB-20-NA
|
Human PDGF Polyclonal
Ab
|
1mg
|
R/RD
|
AF1086
|
Human
PD-1 Antibody
|
100ug
|
R/RD
|
AF2685-SP
|
Human
Phospho-Src Antibody
|
25ug
|
R/RD
|
AF3158
|
Mouse
KLF4 Antibody
|
100ug
|
R/RD
|
AF364-SP
|
Human
CCL17/TARC Antibody
|
25ug
|
R/RD
|
AF5038
|
Human/Mouse
TIAM1 Antibody
|
100ug
|
R/RD
|
ARY003B
|
Proteome
Profiler Human Phospho-Kinase Array Kit
|
1kit
|
R/RD
|
BBE6
|
Human
P-Selectin/CD62P ELISA Kit
|
96T
|
R/RD
|
DADT130
|
Human
ADAMTS13 Quantikine ELISA Kit
|
96T
|
R/RD
|
DCD310
|
Human
CD31/PECAM-1 Quantikine ELISA Kit
|
96T
|
R/RD
|
DCD540
|
Human
ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
|
96T
|
R/RD
|
E-305-025
|
Recombinant
Human Ubiquitin Activating Enzyme (UBE1), CF
|
25UG
|
R/RD
|
E2-607-100
|
Recombinant
Human UbcH2/UBE2H Protein, CF
|
100ug
|
R/RD
|
HAF007
|
Mouse
IgG HRP-conjugated Antibody
|
1ml
|
R/RD
|
HS600B
|
Human
IL-6 Quantikine HS ELISA
|
96T
|
R/RD
|
IC1420P
|
Human
alpha Smooth Muscle Acti
|
100T
|
R/RD
|
KGE011
|
Thromboxane
B2 Parameter Assay Kit
|
96T
|
R/RD
|
LXSAHM-16
|
Human
Magnetic Luminex Screening Assay
|
16
PLEX
|
R/RD
|
MAB1205-SP
|
Human/Mouse/Rat
Phospho-JNK Antibody
|
25ul
|
R/RD
|
MAB1310-SP
|
Human
u-Plasminogen Activator
|
25ug
|
R/RD
|
MAB1778-SP
|
Human
Integrin beta 1/CD29 An
|
25ug
|
R/RD
|
MAB2018
|
Human/Mouse
SOX2 MAb
|
100
ug
|
R/RD
|
MAB2076-SP
|
Human/Mouse/Rat
JNK1/JNK2 Antibody
|
25ug
|
R/RD
|
MAB3389-SP
|
Human/Mouse/Rat
Src Antibody
|
25ug
|
R/RD
|
MAB4259-SP
|
Human
Paxillin Antibody
|
25ug
|
R/RD
|
MAB4467-SP
|
Human
FAK Antibody
|
25ug
|
R/RD
|
MAB61641-SP
|
Human
Phospho-Paxillin (Y31) A
|
25ug
|
R/RD
|
MAB7298-SP
|
Human
Phospho-FAK (S843) Antib
|
25ug
|
R/RD
|
MAB807-SP
|
Human
uPAR Antibody
|
25ug
|
R/RD
|
NB100-65392PE
|
P-Selectin/CD62P
Antibody (Pse
|
100T
|
R/RD
|
NBP1-43415APC
|
Integrin
alpha 2b/CD41 Antibod
|
100T
|
R/RD
|
SK-10
|
Ubiquitin
Conjugation Reaction Buffer Kit
|
1kit
|
R/RD
|
U-100H-10M
|
Recombinant
Human Ubiquitin Protein, CF
|
10mg
|
R/RD
|
VAL601
|
Mouse
IL-1 beta Valukine ELISA Kit
|
96T
|