This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

This antibody is stable when stored at 2-8°C. Do not freeze.

FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

CODEX®
CyTOF®
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.

Clone UCHT-1 recognizes an epitope on human CD3ε.

CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Peptide antigen bound to MHC

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Information on Uniprot.org

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2. Yamada, T. et al. (2003) J Immunol 170:4441-9Article Link
3. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
4. van Dongen, JJM. et al. (1988) Blood 71:603
5. Meuer, SC. et al. (1983) Nature 303:808
6. Beverly, PCL. et al. (1981) Eur. J. Immunol. 11:329
7. Leukocyte Typing III: Code No. 126, 208 and 471
8. Leukocyte Typing VI: Paper No. T3.2
9. Leukocyte Typing VI (1996) Oxford University Press