Anti-IREB2/IRP2 antibody (ab106926)

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商品描述

Product name

Anti-IREB2/IRP2 antibody
See all IREB2/IRP2 primary antibodies
Description

Goat polyclonal to IREB2/IRP2
Host species

Goat
Tested applications

Suitable for: IHC-P,WB
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
Immunogen

Synthetic peptide corresponding to Human IREB2/IRP2 aa 489-503 (internal sequence).
Sequence:

C-SIHYEGSEYKLSHGS

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
- 100 µg at 0.5 mg/ml
Purity

Immunogen affinity purified
Purification notes

ab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality

Polyclonal
Isotype

IgG

Western blot - Anti-IREB2/IRP2 antibody (ab106926)

All lanes : Anti-IREB2/IRP2 antibody (ab106926) at 0.3 µg/ml

Lane 1 : Human Liver lysate in RIPA buffer
Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 105 kDa
Observed band size: 110 kDa

why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

Primary incubation was 1 hour.

An additional band of unknown identity was consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (ab106926)

ab106926 at 3.8 µg/ml staining IREB2 in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.