Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
85-100
1:2
Average %
100
Range %
91-105
1:4
Average %
97
Range %
92-102
1:8
Average %
93
Range %
86-98
回收率:
The recovery of human IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
